李朝军

李朝军 Ph.D

南京大学模式动物研究所 教授，博士生导师

ADSFAEQWER353423413434

细胞分裂调控研究：采用荧光蛋白基因标记技术标记钙离子信号途径中的调节蛋白钙调蛋白及蛋白激酶，将基因导入活细胞内使其表达，观察该蛋白质在细胞周期进程中的空间分布变化，采用FRET方法研究蛋白质在活细胞内的空间和时间的相互作用，采用CALI方法在活细胞内实时淬灭蛋白质的活性研究其功能，使细胞成为一个“微试管”，从而得到与死细胞相比更精确、可靠的结果。目前研究集中研究钙调蛋白calmodulin和Polo like kinase在G2/M转化和胞质分裂的调控研究。

细胞应激的信号传递研究：研究细胞在低氧、血清饥饿、高糖等应激状态下MAPK-Egr-1-GGPPS信号通路的作用。Egr-1是早期应激反应转录因子，受MAPK信号通路的调控。我们发现，Egr-1还可以通过激活GGPPS表达，影响Ras的异戊二烯化修饰，又可反馈影响MAPK的激活。目前正在分子水平、细胞水平、整体水平在研究调控MAPK-Egr-1-对II型糖尿病和肝再生的影响。 ADSFAEQWER353423413434

HGF促进细胞离散的分子机制研究：细胞外基质（ECM）提供了细胞生长的微环境，参与细胞的多种生理过程，并在肿瘤的浸润、迁移、炎症反应等病理过程中发挥重要的作用。我们发现肝细胞生长因子HGF可以通过激活经典的Wnt信号途径，激活MMP7的表达，MMP7通过降解E-cadherin促进了细胞的离散。阻断Wnt/β-catenin的信号可以在动物水平上抑制肝硬化的发生进程和由此引起的门静脉高压。

工作人员：薛斌博士，潘飞燕博士
博士研究生：汪秀星，沈宁，来珊珊，徐娜，刁凡
硕士研究生：孟凡力，丁尧，王江南，姜珊
大学生创新团队：梁金，唐玉霞，应璞，陈卫波，胡东

• 国家重大科学研究计划 “蛋白质整体功能的在体遗传操作新技术新方法研究”子项目：“利用小鼠DNA大片段定向敲除新技术新方法分析黑色素肿瘤抗原蛋白质家族整体功能” （2009-2013）
• 国家重大科学研究计划“心脏发育与血管发生过程中的信号调控机制研究”子项目：“心脏发育的离子通道和应激信号通路的作用” （2007-2012）
• 国家自然科学基金“EGR-1调控胰岛素诱发的PI3K/Akt和MAPK信号传递”（2007-2009）

(通讯作者)： ADFASDFAF23RQ23R

1. Ning Shen, Tao Gong, Jian-Dong Wang, Fan-Li Meng, Long Qiao, Run-Lin Yang, Bin Xue, Fei-Yan Pan, Xiao-Jun Zhou, Hua-Qun Chen, Wen Ning, Chao-Jun Li. Cigarette-smoke induced pulmonary inflammatory responses are mediated by egr-1/ggpps/mapk signaling. Am J Pathol 2011, 178:111–119
2. Ning Shen, Xiao Yu, Fei-Yan Pan, Xiang Gao, Bin Xue, Chao-Jun Li. An early response transcription factor, Egr-1, enhances insulin resistance in type 2 diabetes with chronic hyperinsulinism. Revised by JBC
3. Fei-Yan Pan, Sheng-Zhou Zhang, Na Xu, Fan-Li Meng, Hong-Xia Zhang, Bin Xue, Xiao Han, Chao-Jun Li. β-catenin signaling involves HGF enhanced HepG2 scattering through activating MMP-7 transcription. Histochem. Cell Biol. 2010, 134(3): 285-295
4. Chen H, Wang L, Gong T, Yu Y, Zhu C, Li F, Wang L, Li CJ. EGR-1 regulates HO-1 expression induced by cigarette smoke. Biochem Biophys Res Commun. 2010. 396(2): 388-93
5. Xiao Yu, Weiwei Tao, Fengrong Jiang, Chaojun Li, Jiandie Lin and Chang Liu. Celastrol Attenuates Hypertension-Induced Inflammation and Oxidative Stress in Vascular Smooth Muscle Cells via Induction of Heme Oxygenase-1. Am J Hypertens. 2010. 23: 895-903
6. Sun H, Chen L, Yan G, Wang R, Diao Z, Hu Y, Li C.J. 2009. HOXA10 suppresses p/CAF promoter activity via three consecutive TTAT units in human endometrial stromal cells. Biochem Biophys Res Commun. 379:16-21
7. Min Huang, Jun-Ning Wei, Wan-Xin Peng, Juan Liang, Chun Zhao, Yan Qian, Gu Dai, Jun Yuan, Fei-Yan Pan, Bin Xue, Jia-Hao Sha, CJ Li. 2008. The association of CaM and Hsp70 regulates S-phase arrest and apoptosis in a spatially and temporally dependent manner in human cells. Cell Stress and Chaperones 2008 DOI 10.1007/s12192-008-0088-3
8. Jun Yuan, Guo-Xin Shi, Yue Shao, Gu Dai, Jun-Ning Wei, Donald C. Chang, CJ Li. 2008. Calmodulin bound to stress fibers but not microtubules involves regulation of cell morphology and motility. The International Journal of Biochemistry & Cell Biology 40: 284–293
9. Li CJ, Ning W, Feghali-Bostwick C, Matthay MA and Choi AMK. 2007. MAPK-EGR-1-HSP70 pathway regulates cigarette smoke induced chemokine release in human lung fibroblasts. AJP: Lung Cellular and Molecular Physiology 292(5):L1297-303 (2007)
10. WG Shen, WX. Peng, Y Shao, JF Xu, G Dai, Y Zhang, FY Pan and CJ Li. 2007. Localization and activity of calmodulin is involved in cell–cell adhesion of tumor cells and endothelial cells in response to hypoxic stress. Cell Biol Toxicol, 23(5):323-35
11. Wei-Gan Shen, Wan-Xin Peng, Gu Dai, Jian-Feng Xu, Yu Zhang, CJ Li. 2006. Calmodulin is essential for angiogenesis in response to hypoxic stress in endothelial cells. Cell Biology International 31:126-134
12. Chen J, Dai G, Wang YQ, Wang S, Pan FY, Xue B, Zhao DH, Li CJ. 2006. Polo-like kinase 1 regulates mitotic arrest after UV irradiation through dephosphorylation of p53 and inducing p53 degradation. FEBS Lett. 580(15):3624-30.
13. Zhang SZ, Pan FY, Xu JF, Yuan J, Guo SY, Dai G, Xue B, Shen WG, Wen CJ, Zhao DH, Li CJ. 2005. Knockdown of c-Met by adenovirus-delivered small interfering RNA inhibits hepatocellular carcinoma growth in vitro and in vivo. Mol Cancer Ther. 4(10):1577-84.
14. Ning W, Choi AM, Li CJ. 2005. Carbon monoxide inhibits IL-17-induced IL-6 production through the MAPK pathway in human pulmonary epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289(2): L268-73.
15. Yu YY, Dai G, Pan FY, Chen J, Li CJ. 2005. Calmodulin regulates the post-anaphase reposition of centrioles during cytokinesis. Cell Res. 15(7):548-52.
16. Ning W, Li CJ, Kaminski N, Feghali-Bostwick CA, Alber SM, Di YP, Song RP, Hayashi S, Pinsky DJ, Watkins SC, Pilewski JM, Peters DG, Hogg JC, & Choi AMK. Comprehensive Gene Expression Profiles Reveal Novel Pathways Related to the Pathogenesis of Chronic Obstructive Pulmonary Disease. PNAS, 101(41): 14895-14900. (:equal contribution, Track II)

• Shen N, Gong T, Wang JD, et al. Cigarette-smoke induced pulmonary inflammatory responses are mediated by egr-1/ggpps/mapk signaling. Am J Pathol. 2011;178:111-119.
• Pan FY, Zhang SZ, Xu N, et al. β-catenin signaling involves HGF enhanced HepG2 scattering through activating MMP-7 transcription. Histochem Cell Biol. 2010;134(3):285-295.
• Li CJ, Ning W, Feghali-Bostwick C, et al. MAPK-EGR-1-HSP70 pathway regulates cigarette smoke induced chemokine release in human lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2007;292(5):L1297-L1303.
• Zhang SZ, Pan FY, Xu JF, et al. Knockdown of c-Met by adenovirus-delivered small interfering RNA inhibits hepatocellular carcinoma growth in vitro and in vivo. Mol Cancer Ther. 2005;4(10):1577-1584.
• Ning W, Li CJ, Kaminski N, et al. Comprehensive gene expression profiles reveal novel pathways related to the pathogenesis of chronic obstructive pulmonary disease. Proc Natl Acad Sci USA. 2004;101(41):14895-14900.
• 陈杰, 戴谷, 王玉琴, 等. Polo-like kinase 1 regulates mitotic arrest after UV irradiation through dephosphorylation of p53 and inducing p53 degradation. FEBS Lett. 2006;580(15):3624-3630.
• 黄敏, 魏俊宁, 彭万新, 等. The association of CaM and Hsp70 regulates S-phase arrest and apoptosis in a spatially and temporally dependent manner in human cells. Cell Stress Chaperones. 2009;14(1):1-12.
• Yuan J, Shi GX, Shao Y, et al. Calmodulin bound to stress fibers but not microtubules involves regulation of cell morphology and motility. Int J Biochem Cell Biol. 2008;40(2):284-293.