熊巍

北京脑科学与类脑研究所，研究员，2015年获得国家自然科学基金“优秀青年”基金

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2015-2022    清华大学生命科学学院、清华-麦戈文脑研究所，实验室负责人（Principal Investigator） 

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2013-2015    美国斯克里普斯研究所分子和细胞神经科学系，全职科学家（Staff Scientist） 

2007-2013    美国斯克里普斯研究所分子和细胞神经科学系，博士后（Postdoctoral Research Associate）  导师：Ulrich Müller教授

2006-2007    北京大学分子医学研究所，研究助理（Research Associate）  导师：周专教授

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在五大感觉（视、听、触、嗅、味）系统中，听觉的外周感受机制是研究得最不清楚的，尤其是实现声音解码和编码所用到的机械转导蛋白机器的分子组分和工作机制。熊巍课题组的问题聚焦于：

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• 研究机械转导通道相关蛋白的分子功能以及鉴定新耳聋基因和突变（Auditory transduction）；
• 听觉系统解码语音频率的分子、细胞和神经机制（Auditory processing）；
• 探索遗传疾病的在体生物学治疗方案（Auditory restoration）。

我们的研究理念是以科学假说驱动课题设计，以技术创新拓展研究边界，从而推动概念性和创新性的科学研究。研究手段结合小鼠遗传学、分子生物学、电生理学、荧光成像、小鼠行为学等方法，辅以自行开发的耳蜗组织培养电转体系、声学刺激和记录手段，构建一个比较完整的研究系统，涵盖听觉从受体分子到声音编码识别等科学问题以及相关的转化问题。 ADSFAEQWER353423413434

A) 主题相关通讯作者文章

• Liu, L., Zou, L., Li, K., Hou, H., Hu, Q., Liu, S., Li, J., Song, C., Chen, J., Wang, S., Wang, Y., Li, C., Du, H., Li, J. L., Chen, F., Xu, Z., Sun, W., Sun, Q., and Xiong, W. (2022) Template-independent genome editing in the Pcdh15(av-3j) mouse, a model of human DFNB23 nonsyndromic deafness. Cell Rep 40, 111061
• Li, J., Liu, S., Song, C., Zhu, T., Zhao, Z., Sun, W., Wang, Y., Song, L., and Xiong, W. (2022) Prestin-Mediated Frequency Selectivity Does not Cover Ultrahigh Frequencies in Mice. Neuroscience Bulletin 38, 17
• Yan, Y., Tian, M., Li, M., Zhou, G., Chen, Q., Xu, M., Hu, Y., Luo, W., Guo, X., Zhang, C., Xie, H., Wu, Q. F., Xiong, W., Liu, S., and Guan, J. S. (2022) ASH1L haploinsufficiency results in autistic-like phenotypes in mice and links Eph receptor gene to autism spectrum disorder. Neuron 6273 (21) 01090-4
• Zhang, Y., Lin, G., Wang, Y., Xue, N., Lin, X., Du, T., Xiong, W., and Song, L. (2021) Prestin derived OHC surface area reduction underlies age-related rescaling of frequency place coding. Hearing Research 108406
• Song, C., Li, J., Liu, S., Hou, H., Zhu, T., Chen, J., Liu, L., Jia, Y., and Xiong, W. (2021) An L1 retrotransposon insertion induced deafness mouse model for studying the development and function of the cochlear stria vascularis. Proc Natl Acad Sci U S A 118 (40) e2107933118
• Li, J., Liu, S., Song, C., Hu, Q., Zhao, Z., Deng, T., Wang, Y., Zhu, T., Zou, L., Wang, S., Chen, J., Liu, L., Hou, H., Yuan, K., Zheng, H., Liu, Z., Chen, X., Sun, W., Xiao, B., and Xiong, W. (2021) PIEZO2 mediates ultrasonic hearing via cochlear outer hair cells in mice. Proc Natl Acad Sci U S A 118 (28) e2101207118
• Du, H., Zou, L., Ren, R., Li, N., Li, J., Wang, Y., Sun, J., Yang, J., Xiong, W., and Xu, Z. (2020). Lack of PDZD7 long isoform disrupts ankle-link complex and causes hearing loss in mice. FASEB J 34, 1136-1149
• Wang, Y., Su, F., Wang, S., Yang, C., Tian, Y., Yuan, P., Liu, X., Xiong, W., and Zhang, C. (2019) Efficient implementation of convolutional neural networks in the data processing of two-photon in vivo imaging. Bioinformatics 35, 3208-3210
• Liu, S., Wang, S., Zou, L., Li, J., Song, C., Chen, J., Hu, Q., Liu, L., Huang, P., and Xiong, W. (2019). TMC1 is an essential component of a leak channel that modulates tonotopy and excitability of auditory hair cells in mice. Elife 8, e47441
• Hu, Q., Guo, L., Li, J., Song, C., Yu, L., He, D.Z.Z., and Xiong, W. (2018). Deletion of Kncn Does Not Affect Kinocilium and Stereocilia Bundle Morphogenesis and Mechanotransduction in Cochlear Hair Cells. Front Mol Neurosci 11, 326
• Chen, J., Zhang, X., Li, J., Song, C., Jia, Y., and Xiong, W. (2016). Identification of a Novel ENU-Induced Mutation in Mouse Tbx1 Linked to Human DiGeorge Syndrome. Neural Plast 2016, 5836143
• Xiong, W., Wagner, T., Yan, L., Grillet, N., and Müller, U. (2014) Using injectoporation to deliver genes to mechanosensory hair cells. Nat Protoc 9, no. 10: 2438-49

B) 书和综述

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• Liu, S., Wang, S., Zou, L., and Xiong, W. (2021) Mechanisms in cochlear hair cell mechano-electrical transduction for acquisition of sound frequency and intensity. Cell Mol Life Sci 78, 5083-5094 (Review).
• Xiong, W. and Xu, Z. (2018) Mechanotransduction of the Hair Cell. Springer Singapore

• Liu, L., et al. Template-independent genome editing in the Pcdh15(av-3j) mouse, a model of human DFNB23 nonsyndromic deafness. Cell Rep, 2022, 40: 111061.
• Li, J., et al. Prestin-Mediated Frequency Selectivity Does not Cover Ultrahigh Frequencies in Mice. Neurosci Bull, 2022, 38: 17-28.
• Song, C., et al. An L1 retrotransposon insertion induced deafness mouse model for studying the development and function of the cochlear stria vascularis. PNAS, 2021, 118(40): e2107933118.
• Li, J., et al. PIEZO2 mediates ultrasonic hearing via cochlear outer hair cells in mice. PNAS, 2021, 118(28): e2101207118.
• Liu, S., et al. TMC1 is an essential component of a leak channel that modulates tonotopy and excitability of auditory hair cells in mice. eLife, 2019, 8: e47441.
• Xiong, W., et al. Using injectoporation to deliver genes to mechanosensory hair cells. Nat Protoc, 2014, 9(10): 2438-2449.
• Liu, S., et al. Mechanisms in cochlear hair cell mechano-electrical transduction for acquisition of sound frequency and intensity. Cell Mol Life Sci, 2021, 78: 5083-5094.
• Xiong, W., Xu, Z. Mechanotransduction of the Hair Cell. Springer Singapore, 2018.
• 赵玉林, 等. 遗传性耳聋基因研究进展. 中华耳科学杂志, 2020, 18(3): 567-572.
• Wang, Y., et al. Efficient implementation of convolutional neural networks in the data processing of two-photon in vivo imaging. Bioinformatics, 2019, 35: 3208-3210.