孙坚原

孙坚原，中国科学院生物物理研究所研究员，入选中科院“百人计划”。

孙坚原

联系方式：
电子邮件：jysun(AT)ibp.ac.cn（请将(AT)替换为@，防止垃圾邮件）
电话：010-64888537
传真：010-64871293
邮政编码：100101

• 1993年，博士，华东师范大学
• 1993-1997年，讲师，副教授，华东师范大学生物系脑功能研究室
• 1997-1999年，获马普奖学金，德国马普医学所博士后
• 1999-2002年，美国华盛顿大学麻醉系研究助理
• 2002-2008年，美国德州大学西南医学中心神经科学系研究助理教授
• 2008年至今，入选中科院“百人计划”，生物物理研究所研究员

本课题组主要研究突触囊泡释放的分子生物学和细胞生物学原理。我们结合分子生物学和生物物理学方法，探索控制神经递质精确释放的突触机制。尤其关注的课题是：

1. 怎样的分子生物学机制决定了神经突触钙通道与突触囊泡的耦合？这一耦合是如何调控突触囊泡释放率、释放动力学以及突触可塑性的。
2. 神经递质释放参与神经编码的信号机制。

孙坚原博士在突触传递领域取得了多项重要成果，以下为部分代表性论文：

• Sun J-Y, Pang ZP, Qin D-Q, Fahim A, Adachi R, and Südhof TC. A Dual-Ca2+-Sensor Model for Neurotransmitter Release in a central Synapse. Nature, 450(7170):676-683, 2007.
• Khvotchev M, Dulubova I, Sun J-Y, Dai H, Rizo J, and Sudhof T. Dual Modes of Munc18-1/SNARE Interactions Are Coupled by Functionally Critical Binding to Syntaxin-1 N Terminus. Journal of Neuroscience, 27(45):12147-55, 2007.
• Sun J-Y, Bronk P, Liu X, Han W, Südhof TC. Synapsins regulate use-dependent synaptic plasticity in the calyx of Held by a Ca2+/calmodulin-dependent pathway. Proceedings of the National Academy of Sciences USA, 103(8):2880-5, 2006.
• Pang ZP, Sun J-Y, Rizo J, Maximov A, Südhof TC. Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+-triggered neurotransmitter release. EMBO Journal, 25(10):2039-50, 2006 (co-first author).
• Wu X-S, Sun J-Y, Evers AS, Crowder M, Wu L-G. Isoflurane inhibits transmitter release and the presynaptic action potential. Anesthesiology, 100(3):663-670, 2004.
• Sun J-Y, Wu W, Wu X-S, Jin S-X, Dondzillo A, Wu L-G. Capacitance measurements at the calyx of Held in the medial nucleus of the trapezoid body. Journal of Neuroscience Methods, 134(2):121-131, 2004.
• Sun J-Y, Wu X-S, Wu L-G. Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse. Nature, 417(6888):555-559, 2002 (co-first author).
• Sun J-Y, Wu L-G. Fast Kinetics of exocytosis revealed by simultaneous measurements of presynaptic capacitance and postsynaptic currents at a central synapse. Neuron, 30:171-182, 2001.

（* 表示通讯作者）

孙坚原博士在突触囊泡释放的分子机制研究领域做出了突出贡献，其提出的双钙传感器模型为理解神经递质释放的精确调控提供了重要理论基础，推动了神经科学的发展。

• Sun JY, Pang ZP, Qin DQ, et al. A dual-Ca2+-sensor model for neurotransmitter release in a central synapse. Nature. 2007;450(7170):676-683.
• Khvotchev M, Dulubova I, Sun JY, et al. Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus. J Neurosci. 2007;27(45):12147-12155.
• Sun JY, Bronk P, Liu X, et al. Synapsins regulate use-dependent synaptic plasticity in the calyx of Held by a Ca2+/calmodulin-dependent pathway. Proc Natl Acad Sci USA. 2006;103(8):2880-2885.
• Pang ZP, Sun JY, Rizo J, et al. Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+-triggered neurotransmitter release. EMBO J. 2006;25(10):2039-2050.
• Wu XS, Sun JY, Evers AS, et al. Isoflurane inhibits transmitter release and the presynaptic action potential. Anesthesiology. 2004;100(3):663-670.
• Sun JY, Wu W, Wu XS, et al. Capacitance measurements at the calyx of Held in the medial nucleus of the trapezoid body. J Neurosci Methods. 2004;134(2):121-131.
• Sun JY, Wu XS, Wu LG. Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse. Nature. 2002;417(6888):555-559.
• Sun JY, Wu LG. Fast kinetics of exocytosis revealed by simultaneous measurements of presynaptic capacitance and postsynaptic currents at a central synapse. Neuron. 2001;30:171-182.
• 杨雄里. 神经科学原理. 上海: 上海科学技术出版社; 2014.
• Sudhof TC. The synaptic vesicle cycle. Annu Rev Neurosci. 2004;27:509-547.
• 陆巍, 陈建国. 突触可塑性的分子机制. 生理科学进展. 2008;39(2):97-102.
• Kaeser PS, Regehr WG. Molecular mechanisms for synchronous, asynchronous, and spontaneous neurotransmitter release. Annu Rev Physiol. 2014;76:333-363.